<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Martiny, A. C.</style></author><author><style face="normal" font="default" size="100%">Tai, A. P. K.</style></author><author><style face="normal" font="default" size="100%">Veneziano, D.</style></author><author><style face="normal" font="default" size="100%">Primeau, F. W.</style></author><author><style face="normal" font="default" size="100%">Chisholm, S. W.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Taxonomic resolution, ecotypes and the biogeography of Prochlorococcus</style></title><secondary-title><style face="normal" font="default" size="100%">Environmental Microbiology</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Environ. Microbiol.</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">abundances</style></keyword><keyword><style  face="normal" font="default" size="100%">cyanobacteria</style></keyword><keyword><style  face="normal" font="default" size="100%">diversity</style></keyword><keyword><style  face="normal" font="default" size="100%">evolution</style></keyword><keyword><style  face="normal" font="default" size="100%">Martiny Lab</style></keyword><keyword><style  face="normal" font="default" size="100%">north-atlantic ocean</style></keyword><keyword><style  face="normal" font="default" size="100%">pacific</style></keyword><keyword><style  face="normal" font="default" size="100%">phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">Primeau Modeling Lab</style></keyword><keyword><style  face="normal" font="default" size="100%">quantitative pcr</style></keyword><keyword><style  face="normal" font="default" size="100%">synechococcus</style></keyword><keyword><style  face="normal" font="default" size="100%">time-series</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year><pub-dates><date><style  face="normal" font="default" size="100%">04/2009</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">pub/676</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">4</style></number><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">823-832</style></pages><isbn><style face="normal" font="default" size="100%">1462-2912</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;In order to expand our understanding of the diversity and biogeography of Prochlorococcus ribotypes, we PCR-amplified, cloned and sequenced the 16S/23S rRNA ITS region from sites in the Atlantic and Pacific oceans. Ninety-three per cent of the ITS sequences could be assigned to existing Prochlorococcus clades, although many novel subclades were detected. We assigned the sequences to operational taxonomic units using a graduated scale of sequence identity from 80% to 99.5% and correlated Prochlorococcus diversity with respect to environmental variables and dispersal time between the sites. Dispersal time was estimated using a global ocean circulation model. The significance of specific environmental variables was dependent on the degree of sequence identity used to define a taxon: light correlates with broad-scale diversity (90% cut-off), temperature with intermediate scale (95%) whereas no correlation with phosphate was observed. Community structure was correlated with dispersal time between sample sites only when taxa were defined using the finest sequence similarity cut-off. Surprisingly, the concentration of nitrate, which cannot be used as N source by the Prochlorococcus strains in culture, explains some variation in community structure for some definitions of taxa. This study suggests that the spatial distribution of Prochlorococcus ecotypes is shaped by a hierarchy of environmental factors as well dispersal limitation.&lt;/p&gt;</style></abstract><work-type><style face="normal" font="default" size="100%">Article</style></work-type><accession-num><style face="normal" font="default" size="100%">ISI:000264881300009</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;ISI Document Delivery No.: 428WDTimes Cited: 13Cited Reference Count: 35Cited References:      AHLGREN NA, 2006, ENVIRON MICROBIOL, V8, P441, DOI     10.1111/j.1462-2920.2005.00910.x     BINGHAM J, 2000, BIOINFORMATICS, V16, P660     BOUMAN HA, 2006, SCIENCE, V312, P918, DOI 10.1126/science.1122692     BROWN MV, 2005, ENVIRON MICROBIOL, V7, P1466, DOI     10.1111/j.1462-2920.2005.00835.x     CASEY JR, 2007, GEOPHYS RES LETT, V34, P1     COLWELL RK, 2008, ESTIMATES STAT ESTIM     DESPER R, 2002, J COMPUT BIOL, V9, P687     FELSENSTEIN J, 2006, PHYLIP PHYLOGENY INF     FLORES E, 2005, PHOTOSYNTH RES, V83, P117, DOI 10.1007/s11120-004-5830-9     HUBER T, 2004, BIOINFORMATICS, V20, P2317, DOI     10.1093/bioinformatics/bth226     JOHNSON ZI, 2006, SCIENCE, V311, P1737, DOI 10.1126/science.1118052     KARL DM, 1999, ECOSYSTEMS, V2, P181     KETTLER GC, 2007, PLOS GENET, V3, P2515, ARTN e231     LIU HB, 1997, AQUAT MICROB ECOL, V12, P39     LUDWIG W, 2004, NUCLEIC ACIDS RES, V32, P1363, DOI 10.1093/nar/gkh293     MANTEL N, 1967, CANCER RES, V27, P209     MARTINY AC, 2006, P NATL ACAD SCI USA, V103, P12552, DOI     10.1073/pnas.0601301103     MARTINY JBH, 2006, NAT REV MICROBIOL, V4, P102, DOI 10.1038/nrmicro1341     MOORE LR, 1998, NATURE, V393, P464     MOORE LR, 2002, LIMNOL OCEANOGR, V47, P989     PRIMEAU F, 2005, J PHYS OCEANOGR, V35, P545     ROCAP G, 2002, APPL ENVIRON MICROB, V68, P1180, DOI     10.1128/AEM.68.3.1180-1191.2002     RUSCH DB, 2007, PLOS BIOL, V5, P398, ARTN e77     SCHLOSS PD, 2005, APPL ENVIRON MICROB, V71, P1501, DOI     10.1128/AEM.71.3.1501-1506.2005     SHAW AK, 2008, ENVIRON MICROBIOL, V10, P2200, DOI     10.1111/j.1462-2920.2008.01626.x     SHI T, 2005, MOL BIOL EVOL, V22, P2179, DOI 10.1093/molbev/msi216     STEINBERG DK, 2001, DEEP-SEA RES PT II, V48, P1405     TERBRAAK CJF, 1986, ECOLOGY, V67, P1167     THOMPSON JR, 2002, NUCLEIC ACIDS RES, V30, P2083     VENTER JC, 2004, SCIENCE, V304, P66, DOI 10.1126/science.1093857     WEST NJ, 1999, APPL ENVIRON MICROB, V65, P2585     WHILEY DA, 2005, J CLIN VIROL, V34, P104, DOI 10.1016/j.jcv.2005.02.010     ZINSER ER, 2006, APPL ENVIRON MICROB, V72, P723, DOI     10.1128/AEM.72.1.723-732.2006     ZINSER ER, 2007, LIMNOL OCEANOGR, V52, P2205     ZWIRGLMAIER K, 2008, ENVIRON MICROBIOL, V10, P147, DOI     10.1111/j.1462-2920.2007.01440.xMartiny, Adam C. Tai, Amos P. K. Veneziano, Daniele Primeau, Francois Chisholm, Sallie W.NSF Biological Oceanography, ; NSF C-MORE ; Gordon and Betty Moore Foundation ; University of California ; Danish National Science Foundation ; UK Natural Environment Research Council through the Atlantic Meridional Transect consortium [NER/O/S/2001/00680]We thank Ed Delong and Dennis Ryan for help with phylogenetic analysis. We also thank Jennifer Hughes Martiny, Jorge Frias-Lopez, Mick Follows, John Avise, Steven Allison, Rex Malmstrom and Jason Bragg for many helpful discussions throughout this work, Daniel Sher, Sarah Bagby and Katya Frois-Moniz for detailed comments on the manuscript, Jed Fuhrman and Mark Brown for early access to unpublished ITS sequences and two anonymous reviewers for many helpful comments. Research was supported in part by NSF Biological Oceanography, NSF C-MORE, DOE GTL programme, the Gordon and Betty Moore Foundation (to S.W.C.), University of California, and the Danish National Science Foundation (to A.C.M.). Also, this study was supported by the UK Natural Environment Research Council through the Atlantic Meridional Transect consortium (NER/O/S/2001/00680). This is contribution number 173 of the AMT programme.Wiley-blackwell publishing, incMalden&lt;/p&gt;</style></notes><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;[Martiny, Adam C.; Tai, Amos P. K.; Veneziano, Daniele; Chisholm, Sallie W.] MIT, Dept Civil &amp;amp; Environm Engn, Cambridge, MA 02139 USA. [Martiny, Adam C.; Primeau, Francois] Univ Calif Irvine, Dept Earth Syst Sci, Irvine, CA 92697 USA. [Martiny, Adam C.] Univ Calif Irvine, Dept Ecol &amp;amp; Evolutionary Biol, Irvine, CA 92697 USA.Martiny, AC, MIT, Dept Civil &amp;amp; Environm Engn, Cambridge, MA 02139 USA.amartiny@uci.edu chisholm@mit.edu&lt;/p&gt;</style></auth-address></record></records></xml>